Proteomic and phosphoproteomic profiling reveals melanoma cell responses to T cell attack
By
Giulia Franciosa1,6 Send email to [email protected]
Summary
This study profiles the early proteomic and phosphoproteomic responses of patient-derived melanoma cells when co-cultured with matched autologous tumor-infiltrating lymphocytes (TILs). Using SILAC coupled with Orbitrap Astral DIA mass spectrometry, the researchers were able to distinguish tumor from TIL proteomes without physical cell sorting. The research uncovers new immune reactivity markers and tumor survival mechanisms during active T cell attack, providing a valuable resource for improving cancer immunotherapy.
Source
bskyProteomic and phosphoproteomic profiling reveals melanoma cell responses to T cell attackcell.comKey quotes
· 5 pulledUnderstanding how tumor cells interact with tumor-infiltrating lymphocytes (TILs) is crucial for improving immunotherapy, yet protein-level changes remain largely unexplored.
To distinguish tumor from TIL proteomes without physical sorting, we apply stable isotope labeling by amino acids in cell culture (SILAC) coupled with Orbitrap Astral data-independent acquisition (DIA) mass spectrometry (MS).
This approach enables cell type-specific profiling of protein signaling between patient-derived melanoma and autologous T cells during active tumor attack.
By analyzing these interactions without separating the cells, they uncover new immune reactivity markers and tumor survival mechanisms.
This provides a valuable resource to improve cancer immunotherapy.
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