Genome-wide CRISPR screens identify host factors that promote and restrict HIV infection in primary CD4+ T cells
By
Ujjwal Rathore1,2,18,19 Send email to [email protected]
Summary
This article describes a systematic genome-wide CRISPR activation (CRISPRa) and CRISPR knockout screening approach in primary human CD4+ T cells to identify host factors that either promote or restrict HIV infection. The researchers discovered multiple potent antiviral factors including PI16, PPID, SHISA3, and ITM2A. Notably, PPID was shown to bind the HIV capsid and reduce its nuclear import, revealing a novel mechanism of antiviral activity. The study provides a comprehensive map of host factors involved in HIV infection, with potential implications for developing new therapeutic strategies.
Source
bskyGenome-wide CRISPR screens identify host factors that promote and restrict HIV infection in primary CD4+ T cellscell.comKey quotes
· 4 pulledWe employed orthogonal genome-wide CRISPR activation (CRISPRa) and CRISPR knockout screens in primary CD4+ T cells to discover pro- and anti-HIV host factors systematically.
CRISPRa uncovered multiple potent antiviral factors, including PI16, PPID, SHISA3, and ITM2A.
PI16 interacts with host factors involve
Genome-wide CRISPR activation and knockout screens in primary human CD4+ T cells systematically identify host proviral and antiviral factors modulating HIV infection, including the strongly antiviral factor PPID, which is shown to bind HIV capsid and reduce its nuclear import.
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