Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics
High-speed volumetric imaging of the brain is essential for linking diverse cellular events to tissue-level functions. However, the brain’s structural and dynamic heterogeneity—spanning microns to millimeters and milliseconds to hours—requires imaging techniques with tunable spatiotemporal resolution, flexible 3D sampling, and compatibility with targeted per
