The hidden costs of cryopreservation media: Impacts on cell recovery and experimental reproducibility
Yesterday's bagel, baked twice. Familiar and a bit tough.
Summary
This article discusses how the choice of cryopreservation medium (cryomedium) impacts cell recovery, reproducibility, and downstream experimental results. It argues that while homebrew formulations may seem cost-effective, they carry hidden costs including serum-related variability, preparation time, delayed post-thaw cell loss, and the need for repeated experiments. The piece promotes a webinar that will explore these hidden costs and how serum-containing cryomedia can introduce variability into research.
Key quotes
· 3 pulledCryopreservation is often seen as a routine step, yet the choice of cryomedium can have a major impact on cell recovery, reproducibility, and the reliability of downstream results.
While homebrew formulations may appear cost-effective at first, their true cost can include serum-related variability, preparation time, delayed post-thaw cell loss, and repeated experiments.
We will look beyond immediate post-thaw viability and explore the hidden costs that can influence experimental success.
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