LEAPER 3.0: AlphaFold 3-powered RNA editing platform achieves precise endogenous A-to-I editing
By
Wensheng Wei (魏文胜)1,3,8 Send email to [email protected]
Summary
LEAPER 3.0 is a next-generation RNA-editing platform that uses engineered ADAR-recruiting RNAs (arRNAs) to enable precise and efficient A-to-I RNA editing by leveraging endogenous ADAR enzymes, eliminating the need for exogenous enzyme delivery. The platform integrates AlphaFold 3 structural predictions with biochemical and cellular assays to optimize a dual-bulge arRNA design, achieving improved editing efficiency and precision across diverse ADAR contexts.
Source
Twitter / XLEAPER 3.0: AlphaFold 3-powered RNA editing platform achieves precise endogenous A-to-I editingcell.comKey quotes
· 3 pulledLeveraging endogenous adenosine deaminase (ADAR) enzymes through engineered ADAR-recruiting RNAs (arRNAs) offers a safe, programmable strategy for RNA editing without exogenous enzyme delivery.
Yet an incomplete understanding of ADAR's mechanistic basis has hindered the rational design of arRNAs with improved efficiency and precision.
Here, we present LEAPER 3.0 (leveraging endogenous ADAR for programmable editing of RNA), a next-generation RNA-editing platform that integrates AlphaFold 3 structural predictions with systematic biochemical and cellular assays to define the mole
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