Single-step in vitro reconstitution of the Escherichia coli ribosome mediated by two GTPase factors, EngA and ObgE
When Escherichia coli ribosomes are assembled in vitro, manipulation of incubation temperature and magnesium ion concentration has been an essential procedure, which is a crucial step for the assembly of active large subunits. The present study tackles this issue to develop a single-step procedure, which can be performed in near-physiological conditions, whe
