Genome-wide CRISPR screens identify host factors that promote and restrict HIV infection in primary CD4+ T cells
By
Ujjwal Rathore1,2,18,19 Send email to [email protected]
3d ago· 102 min readenNews
100/100
Golden Brown
Bagelometer↗
Crisp on the outside, thoughtful on the inside. A keeper.
Score100TypenewsSentimentpositive
Summary
This article describes a systematic genome-wide CRISPR activation (CRISPRa) and CRISPR knockout screening approach in primary human CD4+ T cells to identify host factors that either promote or restrict HIV infection. The researchers discovered multiple potent antiviral factors including PI16, PPID, SHISA3, and ITM2A. Notably, PPID was shown to bind the HIV capsid and reduce its nuclear import, revealing a specific mechanism of antiviral action. The study provides a comprehensive map of host factors involved in HIV infection, offering potential targets for therapeutic intervention.
Key quotes
· 4 pulledWe employed orthogonal genome-wide CRISPR activation (CRISPRa) and CRISPR knockout screens in primary CD4+ T cells to discover pro- and anti-HIV host factors systematically.
CRISPRa uncovered multiple potent antiviral factors, including PI16, PPID, SHISA3, and ITM2A.
PI16 interacts with host factors involve
PPID is shown to bind HIV capsid and reduce its nuclear import.
Genome-wide CRISPR activation and knockout screens in primary human CD4+ T cells systematically
identify host proviral and antiviral factors modulating HIV infection, including the
strongly antiviral factor PPID, which is shown to bind HIV capsid and redu